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蔡涛

发布时间:2019-06-24      点击量:207

        博士,北京生命科学研究所测序中心主任,北京市“海聚工程专家人才”。 2006年获北京大学生物信息学博士学位,之后分别在北京生命科学研究所和瑞士洛桑中心医院从事博士后研究。2010年之后任北京生命科学研究所高通量测序中心主任至今。蔡涛博士近年来在非编码RNA,干细胞生物学,基因组学等相关领域发表SCI论文20多篇(发表刊物包括CELL,Cell Stem Cell,PNAS,Bioinformatics等)。


Small RNA transcriptome analysis by parallel single cell small RNA sequencing (PSCSR-seq)

miRNA and other forms of small RNA are now known to regulate many biological processes, and miRNA profiling is already used biomedically for cancer diagnosis, staging, progression, prognosis, and to evaluate responsivity to treatment. However, there is no presently available analytical method capable of dissecting the individual miRNA expression profiles from the highly heterogeneous cells comprising tumor samples. Here, we developed parallel single cell small RNA sequencing (PSCSR-seq), which enables highly sensitive and high-throughput miRNA expression profiling of individual cells. We applied PSCSR-seq to examine the small RNA profiles from large numbers of cultured cancer cells, healthy donor PBMCs, and from a tumor in a melanoma mouse model. We anticipate that PSCSR-seq can be broadly applied for small RNA analysis in cancer research and more generally in life science.